TOXIC EFFECT OF PENTACHLOROPHENOL ON ACTIVITY OF CYTOZOL ENZYMES IN RUMINAL MIXED BACTERIAL POPULATION

N.I. Rusnak, L.G. Kalachnyuk, L.M. Romanyshyna, G.I. Kalachnyuk

It has been shown that the reliable inhibitive effect of light dose (40 µM) of biocide (pentachlorophenol) does affect on activity of all studying enzymes (lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, formate dehydrogenase, malic enzyme, aconitase and fumarase) of mixed bacterial complex in rumen. The level of inhibition of reactions, which catalyze by enzymes pointed above, depends on quantitative and qualitative composition of microbial system formed in appropriate provision of substrates for life-activity. The bringing clinoptilolite media with biocide encourages partial decrease of toxic effect of pentachlorophenol (approximately by 19—47%) on functions of intracellular enzymes of mixed bacterial population in rumen.

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