INVESTIGATION OF THE CHARACTER DEATH OF RAT THYMOCYTES UNDER ACTION OF DIFFERENT XENOBIOTICS

T.O. Kishko, N.P. Dmitrenko, Yu.I. Loboda

In vitro experiments the characteristic death of thymocytes under action of N-nitrosodimethylamine (NDMA), fungicide glyphosate, farmacologycal preparations papaverin and dexamethasone as well as ethanol depending on their concentration and action time was studied. It was shown that influence of investigated xenobiotics except for ethanol thymocytes begin to die afterlatent period during 3 hours. Dose-dependent curves of dynamics of thymocytes death possess the complicate character and have sigmalike shape. The curves showing the dependence of cell death from concentration of xenobiotics have significant individual peculiarities which reflect the presence of thymocytes subpopulations witn different sensitivity to investigated substances. The ethanol induces the linear rise of quantity of death cell. The increasing of ethanol concentration shortens latent period just two times. Dose dependent death of thymocytes under ethanol influence demonstrates the same cell reaction. Summary effect of cytostatic action of NDMA and glyphosate was revealed. Obtained data point to the possibility of thymocytes usage for evaluation of cytotoxic action of individual xenobiotics and their combinations.

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